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Construction and characterization of a BAC library from a gynogenetic channel catfish Ictalurus punctatus

Genetics Selection Evolution volume 35, Article number: 673 (2003) Cite this article

Abstract

A bacterial artificial chromosome (BAC) library was constructed by cloning Hind III-digested high molecular weight DNA from a gynogenetic channel catfish, Ictalurus punctatus, into the vector pBeloBAC11. Approximately 53 500 clones were arrayed in 384-well plates and stored at -80°C (CCBL1), while clones from a smaller insert size fraction were stored at -80°C without arraying (CCBL2). Pulsed-field gel electrophoresis of 100 clones after Not I digestion revealed an average insert size of 165 kb for CCBL1 and 113 kb for CCBL2. Further characterization of CCBL1 demonstrated that 10% of the clones did not contain an insert. CCBL1 provides a 7.2-fold coverage of the channel catfish haploid genome. PCR-based screening demonstrated that 68 out of 74 unique loci were present in the library. This represents a 92% chance to find a unique sequence. These libraries will be useful for physical mapping of the channel catfish genome, and identification of genes controlling major traits in this economically important species.

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Authors and Affiliations

  1. Department of Microbiology, University of Mississippi Medical Center, Jackson, MS, 39216, USA

    Sylvie MA Quiniou, Takayuki Katagiri, Norman W Miller & Melanie Wilson

  2. U.S. Department of Agriculture, Agricultural Research Service, Catfish Genetics Research Unit, Thad Cochran National Warmwater Aquaculture Center, Stoneville, MS, 38776, USA

    Sylvie MA Quiniou, William R Wolters & Geoffrey C Waldbieser

  3. Hubbard Center for Genome Studies, University of New Hampshire, Durham, NH, 03824, USA

    Takayuki Katagiri

Authors
  1. Sylvie MA Quiniou
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  2. Takayuki Katagiri
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  3. Norman W Miller
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  4. Melanie Wilson
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  5. William R Wolters
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  6. Geoffrey C Waldbieser
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Corresponding author

Correspondence to Geoffrey C Waldbieser.

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This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Quiniou, S.M., Katagiri, T., Miller, N.W. et al. Construction and characterization of a BAC library from a gynogenetic channel catfish Ictalurus punctatus. Genet Sel Evol 35, 673 (2003). https://doi.org/10.1186/1297-9686-35-7-673

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  • DOI: https://doi.org/10.1186/1297-9686-35-7-673

Keywords

Genetics Selection Evolution

ISSN: 1297-9686