Email updates

Keep up to date with the latest news and content from Genetics Selection Evolution and BioMed Central.

Open Access Research

Characterization of 35 novel microsatellite DNA markers from the duck (Anas platyrhynchos) genome and cross-amplification in other birds

Yinhua Huang1, Jianfeng Tu12, Xuebo Cheng1, Bo Tang1, Xiaoxiang Hu1, Zhaoliang Liu1, Jidong Feng1, Yankun Lou1, Li Lin1, Ke Xu1, Yulong Zhao1 and Ning Li1*

Author Affiliations

1 State Key Laboratory for Agrobiotechnology, China Agricultural University, Yuanmingyuan, West Road 2, Beijing 100094, PR China

2 College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 300045, PR China

For all author emails, please log on.

Genetics Selection Evolution 2005, 37:455-472  doi:10.1186/1297-9686-37-5-455

The electronic version of this article is the complete one and can be found online at: http://www.gsejournal.org/content/37/5/455


Received:11 January 2005
Accepted:10 February 2005
Published:15 July 2005

© 2005 INRA, EDP Sciences

Abstract

In order to study duck microsatellites, we constructed a library enriched for (CA)n, (CAG)n, (GCC)n and (TTTC)n. A total of 35 pairs of primers from these microsatellites were developed and used to detect polymorphisms in 31 unrelated Peking ducks. Twenty-eight loci were polymorphic and seven loci were monomorphic. A total of 117 alleles were observed from these polymorphic microsatellite markers, which ranged from 2 to 14 with an average of 4.18 per locus. The frequencies of the 117 alleles ranged from 0.02 to 0.98. The highest heterozygosity (0.97) was observed at the CAUD019 microsatellite locus and the lowest heterozygosity (0.04) at the CAUD008 locus, and 11 loci had heterozygosities greater than 0.50 (46.43%). The polymorphism information content (PIC) of 28 loci ranged from 0.04 to 0.88 with an average of 0.42. All the above markers were used to screen the polymorphism in other bird species. Two markers produced specific monomorphic products with the chicken DNA. Fourteen markers generated specific fragments with the goose DNA: 5 were polymorphic and 9 were monomorphic. But no specific product was detected with the peacock DNA. Based on sequence comparisons of the flanking sequence and repeat, we conclude that 2 chicken loci and 14 goose loci were true homologous loci of the duck loci. The microsatellite markers identified and characterized in the present study will contribute to the genetic map, quantitative traits mapping, and phylogenetic analysis in the duck and goose.

Keywords:
duck; microsatellite marker; enriched library; polymorphism; cross-species amplification

Research

(To access the full article, please see PDF)