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Open Access Research

A genome scan for quantitative trait loci affecting the Salmonella carrier-state in the chicken

Pierre Tilquin1, Paul A Barrow2, José Marly3, Frédérique Pitel4, Florence Plisson-Petit4, Philippe Velge3, Alain Vignal4, Philippe V Baret1, Nat Bumstead2 and Catherine Beaumont5*

Author Affiliations

1 Unité de Génétique, Faculté d'ingénierie biologique, agronomique et environnementale, Université catholique de Louvain, Croix du Sud 2 bte 14, B-1348 Louvain-la-Neuve, Belgium

2 Institute for Animal Health, Compton, Berkshire RG20 7NN, UK

3 Station de Pathologie Infectieuse et Immunologie, INRA, 37380 Nouzilly, France

4 Laboratoire de Génétique Cellulaire, INRA, Chemin de Borde-Rouge, Auzeville BP27, 31326 Castanet-Tolosan, France

5 Station de Recherches Avicoles, INRA, 37380 Nouzilly, France

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Genetics Selection Evolution 2005, 37:539-561  doi:10.1186/1297-9686-37-6-539

The electronic version of this article is the complete one and can be found online at: http://www.gsejournal.org/content/37/6/539


Received:5 August 2004
Accepted:2 May 2005
Published:15 September 2005

© 2005 INRA, EDP Sciences

Abstract

Selection for increased resistance to Salmonella colonisation and excretion could reduce the risk of foodborne Salmonella infection. In order to identify potential loci affecting resistance, differences in resistance were identified between the N and 61 inbred lines and two QTL research performed. In an F2 cross, the animals were inoculated at one week of age with Salmonella enteritidis and cloacal swabs were carried out 4 and 5 wk post inoculation (thereafter called CSW4F2 and CSW4F2) and caecal contamination (CAECF2) was assessed 1 week later. The animals from the (N × 61) × N backcross were inoculated at six weeks of age with Salmonella typhimurium and cloacal swabs were studied from wk 1 to 4 (thereafter called CSW1BC to CSW4BC). A total of 33 F2 and 46 backcross progeny were selectively genotyped for 103 and 135 microsatellite markers respectively. The analysis used least-squares-based and non-parametric interval mapping. Two genome-wise significant QTL were observed on Chromosome 1 for CSW2BC and on Chromosome 2 for CSW4F2, and four suggestive QTL for CSW5F2 on Chromosome 2, for CSW5F2 and CSW2BC on chromosome 5 and for CAECF2 on chromosome 16. These results suggest new regions of interest and the putative role of SAL1.

Keywords:
fowl; genetic resistance; Salmonella; carrier-state; SLC11A1

Research

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