Skip to main content
Download PDF

Mapping of quantitative trait loci for flesh colour and growth traits in Atlantic salmon (Salmo salar)

Genetics Selection Evolution volume 42, Article number: 17 (2010) Cite this article

Abstract

Background

Flesh colour and growth related traits in salmonids are both commercially important and of great interest from a physiological and evolutionary perspective. The aim of this study was to identify quantitative trait loci (QTL) affecting flesh colour and growth related traits in an F2 population derived from an isolated, landlocked wild population in Norway (Byglands Bleke) and a commercial production population.

Methods

One hundred and twenty-eight informative microsatellite loci distributed across all 29 linkage groups in Atlantic salmon were genotyped in individuals from four F2 families that were selected from the ends of the flesh colour distribution. Genotyping of 23 additional loci and two additional families was performed on a number of linkage groups harbouring putative QTL. QTL analysis was performed using a line-cross model assuming fixation of alternate QTL alleles and a half-sib model with no assumptions about the number and frequency of QTL alleles in the founder populations.

Results

A moderate to strong phenotypic correlation was found between colour, length and weight traits. In total, 13 genome-wide significant QTL were detected for all traits using the line-cross model, including three genome-wide significant QTL for flesh colour (Chr 6, Chr 26 and Chr 4). In addition, 32 suggestive QTL were detected (chromosome-wide P < 0.05). Using the half-sib model, six genome-wide significant QTL were detected for all traits, including two for flesh colour (Chr 26 and Chr 4) and 41 suggestive QTL were detected (chromosome-wide P < 0.05). Based on the half-sib analysis, these two genome-wide significant QTL for flesh colour explained 24% of the phenotypic variance for this trait.

Conclusions

A large number of significant and suggestive QTL for flesh colour and growth traits were found in an F2 population of Atlantic salmon. Chr 26 and Chr 4 presented the strongest evidence for significant QTL affecting flesh colour, while Chr 10, Chr 5, and Chr 4 presented the strongest evidence for significant QTL affecting growth traits (length and weight). These QTL could be strong candidates for use in marker-assisted selection and provide a starting point for further characterisation of the genetic components underlying flesh colour and growth.

Background

Carotenoid uptake and subsequent deposition in the muscle of fish such as salmon, trout and char is a heritable quantitative trait that is commercially very important for the aquaculture industry [1–3]. Astaxanthin is an expensive ingredient in fish feed (5-10% of feed cost) and muscle deposition of colour in the fish is relatively poor [4, 5]. Market preference for red-fleshed fish has made flesh colour an important trait for breeding goals in Atlantic salmon selection programs. However, at present flesh colour cannot be accurately measured on live adult individuals. Consequently, no within-family selection can be performed and only part of the genetic variation of the trait can be exploited. Marker assisted selection (MAS) using markers linked to quantitative trait loci (QTL) for flesh colour represents an excellent way to improve the efficiency of selection. Heritabilities for flesh colour in Atlantic salmon tend to be low when subjective colour card measurements are used and medium when measurements are based on instrumental methods, with a reported range generally between 0.1 and 0.2 [6, 7].

The extent of genetic control of pigmentation in salmonids has not been conclusively demonstrated. A cross between extremely strong- and weak-coloured populations of Chinook salmon exhibited a phenotypic distribution originally explained by a model involving two loci, each with two alleles [8]. The proposed model could not explain the anomalous red:white ratios among the progeny of one male parent. A recent study has shown that this dataset could be fully explained by a model with one locus and three alleles [9]. In another study [6], a single locus SCAR marker with a relatively strong association to flesh colour in Coho salmon has been identified, suggesting that the genetic control of flesh colour may be controlled by relatively few loci with large effects, rather than a large polygenic effect. A dynamic model of carotenoid metabolism in salmonids, based on ordinary differential equations, has identified the uptake process of carotenoid over the muscle membrane as a potential important source of genetic variation [10]. Given that this model mimics the real situation, the existence of key regulatory sites could possibly suggest the presence of loci with relatively large effects. However this does not necessarily mean that the trait will be regulated via polymorphisms with major effects within the genes encoding these sites.

An F2 population is a useful design to detect loci affecting QTL when two phenotypically distinct populations are crossed [11]. In Atlantic salmon, such populations are relatively rare, and the production of divergent or inbred lines is a long term undertaking due to the long generation interval. However, isolated populations of Atlantic salmon do exist in Norway, and show clear evidence of substantial phenotypic differences from production fish that have been under artificial selection for several generations. The Bleke salmon is a freshwater Atlantic salmon population inhabiting the inner part of the Byglandsfjord in southern Norway. This slow-growing ice age relict was isolated from sea-migrating populations about 9000 years ago because of a waterfall barrier (Vigelandsfoss) [12]. Female Bleke salmon become sexually mature after 4-5 years of freshwater life at a size of about 25 cm fork length [13] compared to that of 70-120 cm in ancestral migratory populations. In 1999, Bleke salmon were crossed to commercial Norwegian salmon selected for fast growth and high colour. The resulting F1 were then crossed to produce an F2 mapping population suitable for the detection of QTL for flesh colour, growth rate and other traits diverging between the parental populations. The aim of our study was to identify QTL affecting flesh colour and growth traits in this F2 population.

Methods

Mapping population

The mapping population consisted of six F2 families that originated from a cross between two divergent populations, the landlocked Byglands Bleke population and a commercial breeding population under selection (Aqua Gen AS). In 1995, three Bleke salmon were crossed with three commercial Norwegian salmon, forming three full-sib families. Five F1 males from one family and five F1 females from another family were subsequently crossed to produce five full-sib F2 families, in addition to a sixth F2 family that was sired by a male from the third F1 family. The pedigree is depicted in Figure 1.

Figure 1
figure 1

Pedigree of the mapping population. Founding generation (P) consisting of Bleke males (Bleke) and Aqua Gen females (AGen).

Full size image

Phenotypic data

F2 progeny were slaughtered at three years of age and had the following traits recorded: length (L), body weight (BW), slaughter weight (SW), and colour (C) in SalmoFanâ„¢ colour units. In addition, Fulton's condition factor (K), a measure of a fish's girth, was calculated as (BW Ă— L3 Ă— 100) [14] and dressing percentage (D%) was calculated as ((BW-SW)/BW Ă— 100). Samples that were paler than the palest colour value (20) on the SalmoFan were given the score 19. Not all the individuals had sufficient gonad developed to be sexed at sampling. For the unsexed progeny, paternal allelic segregation at the microsatellite locus Ssa202DU, known to be tightly linked to the sex-determining locus [15], was used to divide the progeny into males and females. The appropriate marker phase was established from the sexed progeny in each family.

Genotyping

Fifty progeny from each extreme of the colour distribution were selected from three F2 families (8B, 9B and 10B), and all 76 progeny from a fourth family (10A) were selected for genotyping. Corrected values for colour based on the fish size correlation were not used in this selection in order to provide sufficient power for QTL detection for the other traits. Due to differences in progeny numbers between the families, this represented selective genotyping fractions (both extremes) of 44%, 35%, 35% and 100% respectively for families 8B, 9B, 10B and 10A (Table 1). Following the initial QTL analysis, 384 additional individuals were selected from the remaining extremes of the colour distribution from families (8B, 9B and 10B) as well as 384 individuals from two additional families (8A and 9A) for subsequent genotyping at putative QTL.

Table 1 Number of F2 progeny in each family and selective genotyping fractions
Full size table

DNA extraction was carried out from muscle tissue samples using the DNeasy 96 kit (QIAGEN) following the manufacturer's protocol. The majority of microsatellite markers used in this study were chosen from the SALMAP microsatellite map of Atlantic salmon [16], covering all 29 linkage groups (chromosomes). The nomenclature of chromosomes follows that introduced by Philips et al. [17]. In total, 128 informative microsatellite loci were initially genotyped, including duplicated loci amplified from the same primer pair (see additional file 1 for names and female map positions). Following the initial analysis, 23 additional loci were genotyped. The microsatellite markers were distributed across 32 PCR multiplexes that were subsequently combined into 16 multiplexes for capillary electrophoresis. Primer sequences and multiplex information are available on request. Polymerase chain reactions (PCR) were performed in volumes of 5 μL, using 0.25 units of AmpliTaq Gold (Applied Biosystems), 250 μM dNTP mix, 1.5 mM MgCl2, 0.25-1 pmol of each primer (depending on amplification efficiency of each marker in multiplex), 0.25 μL DMSO, and 5 ng DNA template. PCR cycling conditions were 95°C for 10 min, 35 cycles at 94°C for 30 seconds, 54°C for 1 min, and 72°C for 1 min, followed by a final extension at 60°C for 45 min. The lengths of the fluorescent PCR products were determined relative to the LIZ500 size standard (Applied Biosystems) on a 3730 DNA Analyzer (Applied Biosystems), using GeneMapper 4.0 (Applied Biosystems) software for allele calls.

Construction of linkage map

Since samples of the F1 parents were not available, genotypes had to be inferred from the grandparent and progeny genotypes. A custom Visual Basic for Applications program in Excel was used for this task. In situations where it was equally likely for a parental genotype to fit the sire or dam, then, the genotype was arbitrarily applied, the linkage relationship to adjacent markers examined, and finally the parental genotypes reversed if necessary (i.e. if the marker was not linked when it should have been). Separate male and female maps were constructed due to large sex-specific recombination differences observed in salmonids [18]. Marker grouping and initial marker ordering was done with Joinmap 3.0 [19]. A Joinmap input file was made for each mapping parent (in double haploid format), containing information on alleles inherited from that parent only. Marker grouping was performed at a minimum LOD score of 4.0. Following marker grouping, homologous linkage groups from each sire and each dam were integrated into single sex-specific maps. The data was examined for unlikely double recombinants and for inconsistencies in marker order between parents using a custom VBA program in Excel (available by request from the authors). Occurrences of double recombinants over small distances were checked for genotyping errors. After marker orders and potential genotype errors had been verified, the final maps were constructed using Joinmap. The Kosambi mapping function was used.

Interval mapping analyses

Interval mapping using regression methods was applied to two different genetic models: (1) line-cross analysis following Haley et al. [20] assuming founder lines to be fixed for different QTL alleles and (2) half-sib model [21], making no assumptions about the fixation of QTL alleles in the founder lines. In the line-cross model, QTL effects are partitioned into additive and dominance effects. The additive effect was estimated as half the difference between the phenotypic values for homozygotes for the Aqua Gen and Bleke alleles at the QTL, with a positive or a negative sign indicating that the Aqua Gen or the Bleke allele, respectively, increased the value of the trait score. The dominance effect was calculated as the phenotypic deviation of the heterozygotes from the mean of the two homozygotes. GridQTL software [22] was used for QTL analyses. Due to the significant effect of sex on the traits under study, sex was included as a fixed effect for the analysis in both models, based on records of sexed individuals and marker segregation at Ssa202DU. In the initial QTL analysis including four families, male and female mapping parents were analysed separately under the half-sib model. In the subsequent analysis with the larger data set, a joint analysis of male and female mapping parents in the half-sib model was performed by duplicating the dataset prior to analysis, with the designation of parents as sire or dams inverted in the duplicate. In the initial QTL analysis, length was included as a covariate for the analysis of colour, however in the subsequent analysis, body weight was used as the covariate. Full-sib family was fitted as a fixed effect in the line-cross model in the larger dataset (but was omitted in the initial analysis).

P values were calculated for all trait-by-chromosome combinations with the significance of the peak F-statistic (putative QTL) estimated after 10,000 chromosome-wide permutation tests [23]. The chromosomal location of the QTL was taken as the position with the highest F-statistic. Two levels of significance are reported for the detected QTL. A QTL was found to be genome-wide significant if the chromosome-wide significance level was smaller than 0.05 * 29, a Bonferroni correction based on the number of linkage groups examined. QTL that were chromosome-wide significant at P < 0.01 and P < 0.05 but not genome-wide significant were regarded as 'suggestive' QTL. Because this was an initial scan, and also for ease of comparison of the results with those of other studies (as suggested by [24]), correction for multiple traits was not performed. The proportion of phenotypic variance explained by the QTL using the half-sib model was calculated as 4*(1-MSfull/MSreduced) where MSfull is the mean squared error of the full model, accommodating one QTL effect for each informative mapping parent, while MSreduced is the corresponding mean squared error of the reduced model omitting QTL effects [21]. Correction for overestimation of QTL effects due to selective genotyping for flesh colour was not performed due to the different selective genotyping fractions in each family and to the fact that almost all individuals within each family were ultimately genotyped for the four linkage groups that were further investigated. In addition, this correction was not applied for the other traits due to the fact that progeny were only selected from the extremes of the colour distribution and not for these traits (however, the positive correlation between length, weight and colour will mean that some selective genotyping has taken place, and some QTL effect overestimation has occurred). Confidence intervals (CI) were estimated for each genome-wide significant QTL using the bootstrap method [25] and 10,000 iterations.

Results

Phenotypic data analysis

Analysis of raw phenotypic data in the F2 population revealed that all traits exhibited substantial levels of phenotypic variation (Table 2), and strong phenotypic correlations were observed between numerous traits (Table 3). Flesh colour was moderately to strongly correlated to length (0.76), body weight (0.75) and slaughter weight (0.74). Colour was also moderately correlated to K factor (0.60) and weakly correlated to dressing percentage (0.20). There were significant differences in all trait averages between the two sexes (P < 0.001). A total of 6% of all F2 progeny had flesh colour scores below the minimum SalmoFan value of 20, and were therefore given the score 19 for this trait (Figure 2).

Table 2 Phenotypic averages of F2 families. Phenotypic averages and standard deviations (in parentheses) for traits recorded in the six F2 families
Full size table
Table 3 Phenotypic correlations between carcass traits. Phenotypic correlations between carcass traits
Full size table
Figure 2
figure 2

Colour frequency distribution. Frequency distribution of colour scores over the six F2 families.

Full size image

QTL results - Initial genome scan

An initial genome scan was performed using four of the six full-sib families, for the traits flesh colour, body weight and length. Under the across family half-sib model, genome-wide significant QTL were identified for flesh colour on Chr 4, for body weight on Chr 4 and for length on Chr 10 and Chr 4 (Table 4). All QTL were detected in the sire-based analysis. Under the line-cross model, genome-wide significant QTL were identified for flesh colour on Chr 4, for body weight on Chr 5 and Chr 4 and for length on Chr 10 and Chr 4 (Table 4). Numerous additional suggestive QTL were also detected. Genome-wide significance in either model was used as criteria to select chromosomes 10, 5, and 4 for genotyping in additional samples. In addition, suggestive evidence for a colour QTL on Chr 26 under both models was used as criteria for selection of Chr 26 for additional genotyping. Seven hundred and sixty-two additional animals were genotyped for markers on chromosomes 10, 5, 4, and 26. To improve coverage, 23 additional microsatellites were genotyped for chromosomes 26 and 4 (see Additional File 1).

Table 4 Initial QTL analysis using half-sib and line cross models
Full size table

QTL results - Full dataset with the line-cross model

In total, 13 genome-wide significant QTL were detected for all traits using the line-cross model (Table 5). Five QTL were significant at the chromosome-wide P < 0.01 level, and 27 were significant at the chromosome-wide P < 0.05 level (suggestive QTL). Of the 45 significant or suggestive QTL detected, 40 had primarily additive effects, whilst five had larger dominance effects. For flesh colour, three genome-wide significant QTL were detected, two with primarily additive (Chr 26 and Chr 4) and one (Chr 6) with primarily dominance effects. Numerous linkage groups had multiple QTL mapping to them, particularly the strongly correlated length, body weight and slaughter weight traits. Genome-wide significant QTL for colour mapped uniquely to Chr 26 (Figure 3) and Chr 6, and on Chr 4 a genome-wide significant QTL peak (Figure 4) was 53 cM away from genome-wide significant QTL peaks for length and weight (Figure 5). Genome-wide significant QTL for length, body weight and slaughter weight were confirmed on Chr 10 (Figure 6) and Chr 5 (Figure 7). Based on the sign of the additive effect, only three of the 45 QTL were identified where the allele derived from the Bleke line increased the value of the trait score (positive additive effect). 95% QTL confidence intervals were large, covering nearly the entire chromosomes.

Table 5 Quantitative trait loci (QTL) mapped using the F2 line cross regression analysis
Full size table
Figure 3
figure 3

Line-cross and half-sib interval mapping analysis for flesh colour on Chr 26. F-statistic profiles for Chr 26 for both line-cross and half-sib models for flesh colour; diamonds on the top axis represent marker positions; horizontal dashed lines represent genome-wide significance thresholds (P < 0.05) for both line-cross (blue) and half-sib (red) analyses.

Full size image
Figure 4
figure 4

Line-cross and half-sib interval mapping analysis for flesh colour on Chr 4. F-statistic profiles for Chr 4 for both line-cross and half-sib models for flesh colour; diamonds on the top axis represent marker positions; horizontal dashed lines represent genome-wide significance thresholds (P < 0.05) for both line-cross (blue) and half-sib (red) analyses.

Full size image
Figure 5
figure 5

Line-cross and half-sib interval mapping analysis for length and body weight on Chr 4. F-statistic profiles for Chr 4 for both line-cross and half-sib models for length and body weight; diamonds on the top axis represent marker positions; horizontal solid and dashed black lines represent the genome-wide significance thresholds (P < 0.05) for both line-cross and half-sib analyses, respectively.

Full size image
Figure 6
figure 6

Line-cross and half-sib interval mapping analysis for length and body weight on Chr 10. F-statistic profiles for Chr 10 for both line-cross and half-sib models for length and body weight; diamonds on the top axis represent marker positions; horizontal solid and dashed black lines represent the genome-wide significance thresholds (P < 0.05) for both line-cross and half-sib analyses, respectively.

Full size image
Figure 7
figure 7

Line-cross and half-sib interval mapping analysis for length, body weight and slaughter weight on Chr 5. F-statistic profiles for Chr 5 for both line-cross and half-sib models for length and body weight; diamonds on the top axis represent marker positions; horizontal solid and dashed black lines represent the line-cross genome-wide significance threshold (P < 0.05) and half-sib chromosome-wide significance threshold (P < 0.05), respectively.

Full size image

QTL results - Full dataset with the half-sib model

In total, six genome-wide significant QTL were detected for all traits using the half-sib model (Table 6). Of the 41 suggestive QTL identified, 16 QTL were significant at the chromosome-wide P < 0.01 level, and 25 were significant at the P < 0.05 level. Like the line-cross model, numerous linkage groups had multiple QTL mapping to them, with relatively conserved positions for the strongly phenotypically correlated traits. A genome-wide significant QTL for flesh colour mapped to Chr 26 (Figure 3), where no QTL for other traits was detected, and on Chr 4 a genome-wide significant flesh colour QTL peak (Figure 4) was 56 cM away from QTL peaks for length and weight. Together, the two genome-wide significant QTLs for flesh colour on Chr 26 and Chr 4 explained 24% of the phenotypic variance for this trait. Genome-wide significant and suggestive QTL were also detected for length, body weight and slaughter weight on Chr 10 (Figure 6) and Chr 5 (Figure 7). The number of parents showing statistically significant evidence for QTL segregation ranged from one to six (Table 6 and Additional File 2). In most cases, 95% QTL confidence intervals covered nearly the entire chromosome, however the flesh colour QTL interval on Chr 26 was much narrower (38-47 cM).

Table 6 Quantitative trait loci (QTL) mapped using the half-sib regression analysis
Full size table

QTL results - Comparison of two models

All the genome-wide significant QTL mapped using the line-cross model were genome-wide or chromosome-wide significant (P < 0.01) under the half-sib model, with the exceptions of the QTL for flesh colour on Chr 6 and the QTL for length and body weight on Chr 5. Estimates for the amount of phenotypic variance explained by each QTL in the line-cross model were generally much lower than in the half-sib model: 12.6% vs. 3.7% for colour on Chr 26; 11.3% vs. 1.3% for colour on Chr 4; 6.2% vs. 1.4% for body weight on Chr 4; 4.8% vs. 2.3% for length on Chr 10. Numerous suggestive QTL were uniquely detected by both models (Tables 5 and 6).

Discussion

This study used an F2 resource population to identify numerous significant and suggestive QTL for flesh colour, growth and body composition traits in Atlantic salmon. Using line-cross and half-sib regression analyses, genome-wide significant QTL for flesh colour were detected on Chr 6, Chr 26 and Chr 4. Assuming a heritability between 0.1 and 0.2 [6, 7, 26], these QTL could underlie a large portion of the genetic variance for the trait. Salmonids with access to astaxanthin containing diets accumulate carotenoids as they grow, and this accumulation in muscle continues till the fish approach sexual maturity [27]. The ratio of absorbed to non-absorbed carotenoid increases as the fish grows, and as a result, the concentration of fillet astaxanthin normally increases with increasing fish size, which is consistent with the strong positive correlation between fish size and flesh colour observed in this study. Consequently, a large proportion of the observed variance in flesh colour can be explained by body size, reducing the power of QTL detection for this trait. Despite this, highly significant QTL were detected for flesh colour after the inclusion of body weight as a covariate, indicating that there is measurable genetic variation present in this population. Relatively few QTL studies have been carried out on flesh colour traits in salmonids. Araneda et al. [6] identified a dominant SCAR marker associated with colour in Coho salmon (Oncorynchus kisutch), and Houston et al. [28] found suggestive evidence for QTL in Atlantic salmon on chromosomes 16, 18 and 23. None of these QTL reached significance in our study, although chromosomes 18 and 23 reached near chromosome-wide significance. Given the relatively low number of independent loci identified in these studies, and the small number of genome-wide significant QTL found in our study, genetic control of flesh colour in salmonids may be explained by relatively few loci of large effect. However, further validation of the suggestive QTL may reveal that they contribute to a more polygenic effect.

Dahl [12] has reported that the juveniles of the Bleke strain remain in the rivers for two to four years until they reach a length of 12 cm, before migration into the Byglandsfjord, an oligotrophic lake with a poor invertebrate population and no forage fish. In the lake, the Bleke strain exhibits enhanced growth rates, while the maximum fish size generally does not exceed 30 cm and 250 g [12]. After having been landlocked for thousands of years, an adaptation to the poor growing conditions may explain the differences in growth observed between the Bleke and wild fish from the Vosso river. However, the Bleke strain exhibits enhanced growth when transferred to lakes with ample forage fish available [29]. This may suggest that environment rather than genetic effect is more responsible for poor growth. Indeed, ecological factors related to energetics and feeding are almost certainly largely responsible for the establishment of dwarfism in the population, as was documented for Lake Whitefish populations [30]. If this is the case, it represents an important deviation from the assumptions of an F2 population derived from different lines, which are typically under strong selection for particular traits (e.g. [31]). In addition, the trait variance observed in the F2 population, while large (CV = 48.2%, 16% and 15.7% for body weight, total length and K-factor respectively), was of comparable magnitude to other salmon mapping families (45.5%, 17.8% and 9.7% for the same traits) [32] and to outbred full-sib families in other species such as barramundi (Lates calcarifer) (CV = 45.9%, 16.4% and 8.1% for the same traits) [33].

In this study, genome-wide significant QTL for growth and body form traits were found on Chr 10 (BW, L, SW), Chr 5 (BW, L, SW) and Chr 4 (BW, L, SW). Other studies have found evidence for QTL on Chr 4 [32, 34], and QTL have been reported in Arctic charr on linkage groups homologous to Chr 4 and Chr 5 [35]. In addition, numerous linkage groups harbouring suggestive QTL for body weight, length and K-factor were replicated from previous studies. Nevertheless, the large number of different QTL reported for growth traits in Atlantic salmon, in particular body weight, suggests that these traits are highly polygenic (Table 7). Another possible explanation for the different QTL reported for these traits is that different QTL may be segregating in the European and North American populations used in these studies. European and North American Atlantic salmon have been shown to be quite distinct from one another, with FST estimates of 0.27 using microsatellites [36, 37] and 0.33 using allozymes (reviewed in [38]). Therefore it is quite likely that some QTL, such as those affecting body weight, segregate in one subgroup and not in the other.

Table 7 Summary of significant or suggestive body weight QTL in Atlantic salmon reported from this study and the literature
Full size table

The detection of QTL for multiple traits on the same linkage groups (e.g. Chr 4) can be explained by either the linkage of two QTL (one for each trait), or the presence of a single QTL with pleiotropic effects. Reid et al. [34] detected QTL for both body weight and condition factor on five linkage groups in Atlantic salmon, and argued that they may represent different sets of genes due to low genetic correlations reported between the two traits previously. For the colour and 'growth' QTL detected on Chr 4 in this study, there is evidence to suggest that these are two separate QTL, given that the QTL peaks for colour and weight are some distance apart. However, the large, overlapping confidence intervals covering these QTL in both the line-cross and half-sib models means that further analyses will be needed to confirm this. Studies on genetic correlations between flesh colour and growth have been somewhat inconclusive in salmonids. Withler and Beacham [39] have found a moderately positive genetic correlation between final body weight and flesh colour in Coho salmon, however it was not significantly different from zero (0.44 ± 0.48). Other studies have reported stronger evidence for positive genetic correlations between growth and colour in salmonids [2, 40], indicating that the same sets of genes may be involved. An extremely large QTL for IPN resistance explaining nearly all the genetic variance for this trait has been identified on Chr 26 in Atlantic salmon [41], mapping to a similar position to the flesh colour QTL in this study. Although there is little published evidence for a strong genetic correlation between flesh colour and IPN resistance, genotypes at the IPN QTL have been found to be positively correlated to flesh colour (T. Moen, pers. comm.). This suggests the possibility that extreme colour phenotypes represent individuals with alternate IPN QTL alleles due to an undocumented secondary effect of IPN infection on flesh colour. One hypothesis is that a non-lethal infection of a population with IPN could result in the more resistant fish processing or depositing pigment differently to the susceptible fish, resulting in downstream differences in flesh colour that can be explained by the IPN QTL genotype.

Under the line-cross model, the QTL allele with a positive effect on the trait value (additive effect) almost exclusively originated from the commercial line for all traits. This is not surprising given that selection has been performed for a number of generations on growth and body composition traits in this population, while the Bleke population is a natural population subject to environmental selection influence alone. Although the genome-wide significant QTL were generally detected in both the line-cross and half-sib models, a large number of suggestive QTL were uniquely detected by each model. This is likely due to the underlying assumptions of the models. Mapping of QTL using F2 populations is very powerful when the assumption of QTL allele fixation in the founding lines holds true, and is quite robust to limited deviations from this ideal situation [42]. However, when there is a very large reduction of this contrast, the power of detecting the QTL using the line-cross model is substantially reduced [42]. In the extreme case where the lines do not differ with respect to the allele frequency, then the power will be equal to zero. The half-sib model is more general, with no assumption on the number and frequency of QTL alleles in the founder populations and is almost certainly more realistic for the population in this study, since both lines are outbred. In QTL studies performed in divergent pig populations and their crosses, it has been shown that even in these selected populations there is still a considerable amount of genetic variation at loci affecting traits of interest [24]. Other studies in salmonids have also indicated high levels of variability at QTL within strains. In a QTL mapping study for temperature tolerance in Arctic charr [43], it was unexpectedly found that multiple QTL were detected in pure strain parents (Fraser River and Nauyuk Lake). It was hypothesized that, under the assumption that pure strains were almost fixed for alternate alleles, greater effects would have been detected in the male F1 hybrid parent due to segregation of QTL alleles. This was inferred because these strains descend from populations that are adapted to very different thermal regimes.

The extent of QTL variability in the founding lines in our study is also apparent since the half-sib analysis shows that the QTL segregate in only a fraction of the F1 parents. For the flesh colour QTL on Chr 26 and Chr 4, the QTL appeared to be segregating in six and four parents respectively, out of 12 parents in total. For the rest of the suggestive QTL, the number of heterozygous parents ranged between two and four (out of eight for most linkage groups). Interestingly, only the sires appeared to be segregating for colour on Chr 4, which could be explained by the lack of male recombination enabling detection in the sires only, when the underlying variation is actually located some distance away from the nearest marker. One possible weakness of the across-family half-sib analysis as undertaken here is that low QTL heterozygosity in the parents reduces the power of detection [44]. The optimal solution to the analysis of this F2-type dataset could be a combined half-sib/line-cross model, as suggested by Kim et al. [45]. The estimates of the proportion of phenotypic variance explained by the QTL under the line-cross model were substantially smaller than under the half-sib model (the largest QTL for flesh colour explained only 3.7% of the phenotypic variance in the line-cross model vs. 12.6% in the half-sib model). This is probably due to the fact that the F0 lines were outbred and therefore the estimated QTL effects were underestimated [42]. If in such a situation the data are analysed using a line-cross model, the estimated additive effect will be reduced by a fraction (pH - pL), where pH is the frequency in the H line and pL is the frequency in the L line.

Clearly, these results should be further validated with a denser marker map and additional families, since the QTL could only be mapped to relatively broad chromosomal regions. A relatively dense Atlantic salmon SNP chip, recently developed at the Centre for Integrative Genetics (CIGENE) in Norway in collaboration with international partners and containing 5000-7000 polymorphic SNP, may be a useful tool for this purpose. These SNP arrays offer much more efficient genotyping and scoring, and can be relatively inexpensive when coupled with methods such as selective DNA pooling [46, 47]. The increased marker density of this SNP array will not only help close the gaps that are present in the current linkage map, but may facilitate the use of linkage disequilibrium information to further fine-map QTL.

Nevertheless, this study presents useful evidence for QTL of the important commercial and biological trait of flesh colour, and provides additional information on QTL for commercially important growth traits. There is of course a risk that QTL segregating in a resource population like that used in this study may not be found in commercial populations. However, if this should be the case, the QTL identified in the present study still contribute to a better understanding of the genetic control and biological mechanisms underlying the metabolism of dietary pigments in salmon, and the genetic architecture of growth traits in this species.

Conclusions

A large number of significant and suggestive QTL for flesh colour and growth traits were found in an F2 cross between a landlocked and a commercial strain of Atlantic salmon. Chr 26 and Chr 4 presented the strongest evidence for significant QTL affecting flesh colour, while Chr 10, Chr 5 and Chr 4 presented the strongest evidence for significant QTL affecting growth traits (length and weight). These QTL could be strong candidates for use in marker-assisted selection and may provide further insight into the genetic control of flesh colour and growth traits in this species.

References

  1. Gjerde B, Schaeffer LR: Body traits in rainbow trout: II. Estimates of heritabilities and of phenotypic and genetic correlations. Aquaculture. 1989, 80: 25-44. 10.1016/0044-8486(89)90271-8.

    Article  Google Scholar 

  2. Quinton CD, McMillan I, Glebe BD: Development of an Atlantic salmon (Salmo salar) genetic improvement program: Genetic parameters of harvest body weight and carcass quality traits estimated with animal models. Aquaculture. 2005, 247: 211-217. 10.1016/j.aquaculture.2005.02.030.

    Article  Google Scholar 

  3. Iwamoto RN, Myers JM, Hershberger WK: Heritability and genetic correlations for flesh coloration in pen-reared coho salmon. Aquaculture. 1990, 86: 181-190. 10.1016/0044-8486(90)90111-Y.

    Article  Google Scholar 

  4. Torissen OJ, Hardy RW, Shearer KD: Pigmentation of salmonids- carotenoid deposition and metabolism. Crit Rev Aquat Sci. 1989, 1: 209-225.

    Google Scholar 

  5. Nickell DC, Bromage NR: The effect of timing and duration of feeding astaxanthin on the development and variation of fillet colour and efficiency of pigmentation in rainbow trout (Oncorhynchus mykiss). Aquaculture. 1998, 169: 233-246. 10.1016/S0044-8486(98)00385-8.

    Article  CAS  Google Scholar 

  6. Araneda C, Neira R, Iturra P: Identification of a dominant SCAR marker associated with colour traits in Coho salmon (Oncorhynchus kisutch). Aquaculture. 2005, 247: 67-73. 10.1016/j.aquaculture.2005.02.028.

    Article  CAS  Google Scholar 

  7. Gjerde B, Gjedrem T: Estimates of phenotypic and genetic parameters for carcass traits in Atlantic salmon and rainbow trout. Aquaculture. 1984, 36: 97-110. 10.1016/0044-8486(84)90057-7.

    Article  Google Scholar 

  8. Withler RE: Genetic variation in carotenoid pigment deposition in the red-fleshed and white-fleshed Chinook salmon (Oncorhynchus tshawytscha) of Quesnel River, British Columbia. Can J Genet Cytol. 1986, 28: 587-594.

    Article  Google Scholar 

  9. Rajasingh H, Gjuvsland AB, Vage DI, Omholt SW: When parameters in dynamic models become phenotypes: A case study on flesh pigmentation in the Chinook salmon (Oncorhynchus tshawytscha). Genetics. 2008, 179: 1113-1118. 10.1534/genetics.108.087064.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  10. Rajasingh H, Oyehaug L, Vage D, Omholt S: Carotenoid dynamics in Atlantic salmon. BMC Biology. 2006, 4: 10-10.1186/1741-7007-4-10.

    Article  PubMed Central  PubMed  Google Scholar 

  11. Soller M, Brody T, Genizi A: On the power of experimental designs for the detection of linkage between marker loci and quantitative loci in crosses between inbred lines. Theor Appl Genet. 1976, 47: 35-39. 10.1007/BF00277402.

    Article  CAS  PubMed  Google Scholar 

  12. Dahl K: The Blege or dwarf-salmon. Skr Utg Av Det Norske Videnskabsakad, 1927, Oslo. 1928

    Google Scholar 

  13. Barlaup BT, Kleiven E, Christensen H, Kile H, Martinsen BO, Vethe A: Bleka I Byglandsforden--bestandsstatus og tiltak for økt naturlig rekruttering. 2005, Direktoratet for naturforvaltning. Trondheim, 72:

    Google Scholar 

  14. Rikardsen AH, Elliott JM: Variations in juvenile growth, energy allocation and life-history strategies of two populations of Arctic charr in North Norway. J Fish Biol. 2000, 56: 328-346. 10.1111/j.1095-8649.2000.tb02110.x.

    Article  Google Scholar 

  15. Artieri CG, Mitchell LA, Ng SHS, Parisotto SE, Danzmann RG, Hoyheim B, Phillips RB, Morasch M, Koop BF, Davidson WS: Identification of the sex-determining locus of Atlantic salmon (Salmo salar) on chromosome 2. Cytogenet Genome Res. 2006, 112: 152-159. 10.1159/000087528.

    Article  CAS  PubMed  Google Scholar 

  16. ASalBase. [http://www.asalbase.org]

  17. Phillips R, Keatley K, Morasch M, Ventura A, Lubieniecki K, Koop B, Danzmann R, Davidson W: Assignment of Atlantic salmon (Salmo salar) linkage groups to specific chromosomes: Conservation of large syntenic blocks corresponding to whole chromosome arms in rainbow trout (Oncorhynchus mykiss). BMC Genet. 2009, 10: 46-10.1186/1471-2156-10-46.

    Article  PubMed Central  PubMed  Google Scholar 

  18. Moen T, Hoyheim B, Munck H, Gomez-Raya L: A linkage map of Atlantic salmon (Salmo salar) reveals an uncommonly large difference in recombination rate between the sexes. Anim Genet. 2004, 35: 81-92. 10.1111/j.1365-2052.2004.01097.x.

    Article  CAS  PubMed  Google Scholar 

  19. Stam P, Van Ooijen JW: JoinMap Version 2.0: Software for the Calculation of Genetic Linkage Maps. 1995, CPRO-DLO, Wageningen

    Google Scholar 

  20. Haley CS, Knott SA, Elsen JM: Mapping Quantitative Trait Loci in Crosses Between Outbred Lines Using Least Squares. Genetics. 1994, 136: 1195-1207.

    PubMed Central  CAS  PubMed  Google Scholar 

  21. Knott SA, Elsen JM, Haley CS: Methods for multiple marker mapping of quantitative trait loci in half-sib populations. Theor Appl Genet. 1996, 93: 71-80. 10.1007/BF00225729.

    Article  CAS  PubMed  Google Scholar 

  22. Seaton G, Haley CS, Knott SA, Kearsey M, Visscher PM: QTL Express: Mapping quantitative trait loci in simple and complex pedigrees. Bioinformatics. 2002, 18: 339-340. 10.1093/bioinformatics/18.2.339.

    Article  CAS  PubMed  Google Scholar 

  23. Churchill GA, Doerge RW: Empirical threshold values for quantitative trait mapping. Genetics. 1994, 138: 963-971.

    PubMed Central  CAS  PubMed  Google Scholar 

  24. de Koning DJ, Janss LLG, Rattink AP, van Oers PAM, de Vries BJ, Groenen MAM, van der Poel JJ, de Groot PN, Brascamp EW, van Arendonk JAM: Detection of Quantitative Trait Loci for Backfat Thickness and Intramuscular Fat Content in Pigs (Sus scrofa). Genetics. 1999, 152: 1679-1690.

    PubMed Central  CAS  PubMed  Google Scholar 

  25. Visscher PM, Thompson R, Haley CS: Confidence intervals in QTL mapping by bootstrapping. Genetics. 1996, 143: 1013-1020.

    PubMed Central  CAS  PubMed  Google Scholar 

  26. Norris AT, Cunningham EP: Estimates of phenotypic and genetic parameters for flesh colour traits in farmed Atlantic salmon based on multiple trait animal model. Livest Prod Sci. 2004, 89: 209-222. 10.1016/j.livprodsci.2004.02.010.

    Article  Google Scholar 

  27. Bjerkeng B, Storebakken T, Liaaen-Jensen S: Pigmentation of rainbow trout from start feeding to sexual maturation. Aquaculture. 1992, 108: 333-346. 10.1016/0044-8486(92)90117-4.

    Article  CAS  Google Scholar 

  28. Houston RD, Bishop SC, Hamilton A, Guy DR, Tinch AE, Taggart JB, Derayat A, McAndrew BJ, Haley CS: Detection of QTL affecting harvest traits in a commercial Atlantic salmon population. Anim Genet. 2009, 40: 753-755. 10.1111/j.1365-2052.2009.01883.x.

    Article  CAS  PubMed  Google Scholar 

  29. Jensen KW: Reliktlaks. Sportsfiskerens leksikon. Edited by: Jensen KW. 1984, Oslo

    Google Scholar 

  30. Trudel M, Tremblay A, Schetagne R, Rasmussen JB: Why are dwarf fish so small? An energetic analysis of polymorphism in lake whitefish (Coregonus clupeaformis). Can J Fish Aquat Sci. 2001, 58: 394-405. 10.1139/cjfas-58-2-394.

    Article  Google Scholar 

  31. Nones K, Ledur MC, Ruy DC, Baron EE, Melo CMR, Moura ASAMT, Zanella EL, Burt DW, Coutinho LL: Mapping QTLs on chicken chromosome 1 for performance and carcass traits in a broiler Ă— layer cross. Anim Genet. 2006, 37: 95-100. 10.1111/j.1365-2052.2005.01387.x.

    Article  CAS  PubMed  Google Scholar 

  32. Boulding EG, Culling M, Glebe B, Berg PR, Lien S, Moen T: Conservation genomics of Atlantic salmon: SNPs associated with QTLs for adaptive traits in parr from four trans-Atlantic backcrosses. Heredity. 2008, 101: 381-391. 10.1038/hdy.2008.67.

    Article  CAS  PubMed  Google Scholar 

  33. Wang C, Lo L, Zhu Z, Yue G: A genome scan for quantitative trait loci affecting growth-related traits in an F1 family of Asian seabass (Lates calcarifer). BMC Genomics. 2006, 7: 274-10.1186/1471-2164-7-274.

    Article  PubMed Central  PubMed  Google Scholar 

  34. Reid DP, Szanto A, Glebe B, Danzmann RG, Ferguson MM: QTL for body weight and condition factor in Atlantic salmon (Salmo salar): comparative analysis with rainbow trout (Oncorhynchus mykiss) and Arctic charr (Salvelinus alpinus). Heredity. 2005, 94: 166-172. 10.1038/sj.hdy.6800590.

    Article  CAS  PubMed  Google Scholar 

  35. Moghadam H, Poissant J, Fotherby H, Haidle L, Ferguson M, Danzmann R: Quantitative trait loci for body weight, condition factor and age at sexual maturation in Arctic charr (Salvelinus alpinus): comparative analysis with rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar). Mol Genet Genomics. 2007, 277: 647-661. 10.1007/s00438-007-0215-3.

    Article  CAS  PubMed  Google Scholar 

  36. King TL, Kalinowski ST, Schill WB, Spidle AP, Lubinski BA: Population structure of Atlantic salmon (Salmo salar L.): a range-wide perspective from microsatellite DNA variation. Mol Ecol. 2001, 10: 807-821. 10.1046/j.1365-294X.2001.01231.x.

    Article  CAS  PubMed  Google Scholar 

  37. King TL, Verspoor E, Spidle AP, Gross R, Phillips RB, Koljonen M-L, Sanchez JA, Morrison CL: Biodiversity and Population Structure. The Atlantic Salmon - Genetics, Conservation and Management. Edited by: Verspoor E, Stradmeyer L, Nielsen JL. 2007, Blackwell Publishing Ltd.

    Google Scholar 

  38. Verspoor E, Beardmore JA, Consuegra S, GarcĂ­a de LeĂ¡niz C, Hindar K, Jordan WC, Koljonen ML, Mahkrov AA, Paaver T, SĂ¡nchez JA: Population structure in the Atlantic salmon: insights from 40 years of research into genetic protein variation. J Fish Biol. 2005, 67: 3-54. 10.1111/j.0022-1112.2005.00838.x.

    Article  CAS  Google Scholar 

  39. Withler RE, Beacham TD: Genetic variation in body weight and flesh colour of the coho salmon (Oncorhynchus kisutch) in British Columbia. Aquaculture. 1994, 119: 135-148. 10.1016/0044-8486(94)90170-8.

    Article  Google Scholar 

  40. Rye M, Gjerde B: Phenotypic and genetic parameters of body composition traits and flesh colour in Atlantic salmon, Salmo salar L. Aquac Res. 1996, 27: 121-133. 10.1111/j.1365-2109.1996.tb00976.x.

    Article  Google Scholar 

  41. Houston RD, Haley CS, Hamilton A, Guy DR, Tinch AE, Taggart JB, McAndrew BJ, Bishop SC: Major Quantitative Trait Loci Affect Resistance to Infectious Pancreatic Necrosis in Atlantic Salmon (Salmo salar). Genetics. 2008, 178: 1109-1115. 10.1534/genetics.107.082974.

    Article  PubMed Central  PubMed  Google Scholar 

  42. Alfonso L, Haley CS: Power of different F2 schemes for QTL detection in livestock. Anim Sci. 1998, 66: 1-8. 10.1017/S135772980000878X.

    Article  Google Scholar 

  43. Somorjai IML, Danzmann RG, Ferguson MM: Distribution of temperature tolerance quantitative trait loci in arctic charr (Salvelinus alpinus) and inferred homologies in rainbow trout (Oncorhynchus mykiss). Genetics. 2003, 165: 1443-1456.

    PubMed Central  CAS  PubMed  Google Scholar 

  44. Massault C, Bovenhuis H, Haley C, De Koning DJ: QTL mapping designs for species in aquaculture. Aquaculture. 2007, 272: S289-S289. 10.1016/j.aquaculture.2007.07.134.

    Article  Google Scholar 

  45. Kim J-J, Zhao H, Thomsen H, Rothschild MF, Dekkers JCM: Combined line-cross and half-sib QTL analysis of crosses between outbred lines. Genet Res. 2005, 85: 235-248. 10.1017/S0016672305007597.

    Article  CAS  PubMed  Google Scholar 

  46. Darvasi A, Soller M: Selective DNA pooling for determination of linkage between a molecular marker and a quantitative trait locus. Genetics. 1994, 138: 1365-1373.

    PubMed Central  CAS  PubMed  Google Scholar 

  47. Macgregor S, Zhao ZZ, Henders A, Martin NG, Montgomery GW, Visscher PM: Highly cost-efficient genome-wide association studies using DNA pools and dense SNP arrays. Nucl Acids Res. 2008, 36: e35-10.1093/nar/gkm1060.

    Article  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgements

This study was funded by the Norwegian Research Council (177036/S10) who provided access to the SALBANK samples. Genomar AS and AKVAFORSK (Averøy) produced the families and performed the trait recording. We also thank Bjørn Høyheim and Anna Sonesson for storage and registration of samples and data, Roy Danzmann for providing microsatellite primer sequences, Hege Munck and Katrine HĂ¥nes for genotyping assistance and Tone Hæg Lindholm for DNA extraction and genotyping assistance.

Author information

Authors and Affiliations

  1. Nofima Marin, P.O. Box 5010, 1432, Ă…s, Norway

    Matthew Baranski & Thomas Moen

  2. Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, P.O. Box 5003, 1432, Ă…s, Norway

    Dag Inge VĂ¥ge

  3. The Centre for Integrative Genetics (CIGENE), Norwegian University of Life Sciences, P.O. Box 5003, 1432, Ă…s, Norway

    Matthew Baranski, Thomas Moen & Dag Inge VĂ¥ge

  4. Aqua Gen AS, Postboks 1240, Pirsenteret, 7462, Trondheim, Norway

    Thomas Moen

Authors
  1. Matthew Baranski
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Thomas Moen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dag Inge VĂ¥ge
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Matthew Baranski.

Additional information

Competing interests

The authors declare that they have no competing interests.

Authors' contributions

DIV coordinated and supervised the study. MB performed the laboratory work with assistance from TM, conducted the data analyses and wrote the manuscript with contributions from TM and DIV. All authors read and approved the final manuscript.

Electronic supplementary material

12711_2009_2446_MOESM1_ESM.XLS

Additional file 1: Markers used and map positions. List of markers and female map positions used in this study. (XLS 32 KB)

12711_2009_2446_MOESM2_ESM.XLS

Additional file 2: QTL effects for segregating parents. Allele substitution effects and absolute t-values for segregating parents (QTL at least chromosome-wide significant at P < 0.01). (XLS 28 KB)

Authors’ original submitted files for images

Rights and permissions

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and permissions

About this article

Cite this article

Baranski, M., Moen, T. & VĂ¥ge, D.I. Mapping of quantitative trait loci for flesh colour and growth traits in Atlantic salmon (Salmo salar). Genet Sel Evol 42, 17 (2010). https://doi.org/10.1186/1297-9686-42-17

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/1297-9686-42-17

Keywords

Genetics Selection Evolution

ISSN: 1297-9686